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Repeat-based holocentromeres influence genome architecture and karyotype evolution.
Hofstatter, Paulo G; Thangavel, Gokilavani; Lux, Thomas; Neumann, Pavel; Vondrak, Tihana; Novak, Petr; Zhang, Meng; Costa, Lucas; Castellani, Marco; Scott, Alison; Toegelová, Helena; Fuchs, Joerg; Mata-Sucre, Yennifer; Dias, Yhanndra; Vanzela, André L L; Huettel, Bruno; Almeida, Cicero C S; Simková, Hana; Souza, Gustavo; Pedrosa-Harand, Andrea; Macas, Jiri; Mayer, Klaus F X; Houben, Andreas; Marques, André.
Afiliação
  • Hofstatter PG; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Thangavel G; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Lux T; Plant Genome and Systems Biology, German Research Center for Environmental Health, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany.
  • Neumann P; Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Ceské Budejovice 37005, Czech Republic.
  • Vondrak T; Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Ceské Budejovice 37005, Czech Republic; Faculty of Science, University of South Bohemia, Ceské Budejovice 37005, Czech Republic.
  • Novak P; Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Ceské Budejovice 37005, Czech Republic.
  • Zhang M; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Costa L; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Centre of Biosciences, Federal University of Pernambuco, Recife, Pernambuco 50670-901, Brazil.
  • Castellani M; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Scott A; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Toegelová H; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, Olomouc 779 00, Czech Republic.
  • Fuchs J; Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Saxony-Anhalt 06466, Germany.
  • Mata-Sucre Y; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Centre of Biosciences, Federal University of Pernambuco, Recife, Pernambuco 50670-901, Brazil.
  • Dias Y; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Centre of Biosciences, Federal University of Pernambuco, Recife, Pernambuco 50670-901, Brazil.
  • Vanzela ALL; Laboratory of Cytogenetics and Plant Diversity, State University of Londrina, 86097-570 Paraná, Brazil.
  • Huettel B; Max Planck Genome-Centre Cologne, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany.
  • Almeida CCS; School of Agronomical Sciences, Campus Arapiraca, Federal University of Alagoas, Arapiraca 57309-005, Brazil.
  • Simková H; Institute of Experimental Botany of the Czech Academy of Sciences, Centre of Plant Structural and Functional Genomics, Olomouc 779 00, Czech Republic.
  • Souza G; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Centre of Biosciences, Federal University of Pernambuco, Recife, Pernambuco 50670-901, Brazil.
  • Pedrosa-Harand A; Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Centre of Biosciences, Federal University of Pernambuco, Recife, Pernambuco 50670-901, Brazil.
  • Macas J; Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Ceské Budejovice 37005, Czech Republic.
  • Mayer KFX; Plant Genome and Systems Biology, German Research Center for Environmental Health, Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany; School of Life Sciences Weihenstephan, Technical University of Munich, Alte Akademie 8, 85354 Freising, Germany.
  • Houben A; Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Saxony-Anhalt 06466, Germany.
  • Marques A; Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, Cologne, NRW 50829, Germany. Electronic address: amarques@mpipz.mpg.de.
Cell ; 185(17): 3153-3168.e18, 2022 08 18.
Article em En | MEDLINE | ID: mdl-35926507
The centromere represents a single region in most eukaryotic chromosomes. However, several plant and animal lineages assemble holocentromeres along the entire chromosome length. Here, we compare genome organization and evolution as a function of centromere type by assembling chromosome-scale holocentric genomes with repeat-based holocentromeres from three beak-sedge (Rhynchospora pubera, R. breviuscula, and R. tenuis) and their closest monocentric relative, Juncus effusus. We demonstrate that transition to holocentricity affected 3D genome architecture by redefining genomic compartments, while distributing centromere function to thousands of repeat-based centromere units genome-wide. We uncover a complex genome organization in R. pubera that hides its unexpected octoploidy and describe a marked reduction in chromosome number for R. tenuis, which has only two chromosomes. We show that chromosome fusions, facilitated by repeat-based holocentromeres, promoted karyotype evolution and diploidization. Our study thus sheds light on several important aspects of genome architecture and evolution influenced by centromere organization.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Centrômero / Cyperaceae Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Centrômero / Cyperaceae Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha