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Ethylenediaminetetraacetic acid (EDTA) enhances cAMP production in human TDAG8-expressing cells.
Deai, Masahito; Oya, Rin; Saso, Naosi; Tanaka, Asahi; Uchida, Izumi; Miyake, Yuta; Tachihara, Ryo; Otsugu, Miku; Mine, Ayumi; Sato, Koichi; Tomura, Hideaki.
Afiliação
  • Deai M; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Oya R; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Saso N; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Tanaka A; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Uchida I; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Miyake Y; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Tachihara R; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Otsugu M; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Mine A; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan.
  • Sato K; Laboratory of Signal Transduction, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, 371-8512, Japan.
  • Tomura H; Laboratory of Cell Signaling Regulation, Department of Life Sciences, School of Agriculture, Meiji University, Kawasaki, 214-8571, Japan; Institute of Endocrinology, Meiji University, Kawasaki, 214-8571, Japan. Electronic address: tomurah@meiji.ac.jp.
Biochem Biophys Res Commun ; 626: 15-20, 2022 10 20.
Article em En | MEDLINE | ID: mdl-35964552
ABSTRACT
Ethylenediaminetetraacetic acid (EDTA) is a chelating agent that binds tightly to metal ions. We found that cAMP response element (CRE)-driven promoter activity by protons was enhanced by EDTA in human T-cell death-associated gene 8 (TDAG8)-overexpressed HEK293T cells. The enhancing action by EDTA was also detected by proton-induced cAMP production that is located upstream from the CRE-driven promoter activity even at physiological proton concentration pH7.4. The proton-induced CRE-driven promoter activity was not enhanced by other chelating agents, ethylene glycol tetraacetic acid (EGTA) and sodium citrate. The enhanced CRE-driven promoter activity by EDTA was not attenuated by increasing the extracellular calcium ion concentration. These results indicate that the EDTA-enhancing action may not be due to its chelating action but might rather be another EDTA-specific effect. Enhanced cAMP production by EDTA was also detected in a human leukemia cell line HL-60, in which TDAG8 and OGR1 (ovarian cancer G-protein-coupled receptor 1) were endogenously expressed, suggesting that the medical use of EDTA would influence the physiological and pathophysiological functions of hematopoietic cells.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Prótons / AMP Cíclico Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Prótons / AMP Cíclico Limite: Humans Idioma: En Revista: Biochem Biophys Res Commun Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão