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Rapid, Low-Complexity, Simultaneous Bacterial Group Identification and Antimicrobial Susceptibility Testing Performed Directly on Positive Blood Culture Bottles Using Chromogenic Agar.
Robberts, F J Lourens; Owusu-Ofori, Alex; Oduro, George; Gyampomah, Thomas K; Marles, Nisha; Fox, Anne T; Chenoweth, Josh G; Schully, Kevin L; Clark, Danielle V.
Afiliação
  • Robberts FJL; Independent Consultant, Stellenbosch, South Africa.
  • Owusu-Ofori A; Department of Clinical Microbiology, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
  • Oduro G; Komfo Anokye Teaching Hospital, Kumasi, Ghana.
  • Gyampomah TK; Komfo Anokye Teaching Hospital, Kumasi, Ghana.
  • Marles N; Komfo Anokye Teaching Hospital, Kumasi, Ghana.
  • Fox AT; American Society for Microbiology, Global Public Health Programs, Washington, District of Columbia.
  • Chenoweth JG; Naval Medical Research Unit-3 Ghana Detachment, Accra, Ghana.
  • Schully KL; The Austere Environments Consortium for Enhanced Sepsis Outcomes, The Henry M. Jackson Foundation for the Advancement of Military Medicine Inc., Bethesda, Maryland.
  • Clark DV; The Austere Environments Consortium for Enhanced Sepsis Outcomes, Naval Medical Research Centre, Fort Detrick, Maryland.
Am J Trop Med Hyg ; 107(6): 1302-1307, 2022 12 14.
Article em En | MEDLINE | ID: mdl-36375459
ABSTRACT
The use of positive blood culture bottles for direct disk diffusion susceptibility testing (dDD), together with chromogenic culture limited to groups of pathogens for antimicrobial susceptibility testing interpretation may provide a means for laboratories-in-development to introduce rapid abbreviated blood culture testing. We assessed the performance of dDD on Chromatic MH agar using contrived positive blood culture bottles and compared findings with current standard practice. Furthermore, we characterized the growth of 24 bacterial and 3 yeast species on Chromatic MH agar with the aid of rapid spot tests for same-day identification. The coefficient of variation for reproducibility of dDD of four reference strains in 4 to 10 replicates (238 data points) ranged from 0% to 16.3%. Together with an additional 10 challenge isolates, the overall categorical agreement was 91.7% (351 data points). The following bacteria were readily identifiable cream/white Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes; turquoise Streptococcus agalactiae, enterococci, Listeria monocytogenes; mauve Escherichia coli, Shigella sonnei, Citrobacter freundii; dark-blue Klebsiella and Enterobacter; green Pseudomonas aeruginosa; and brown Proteus. Clear colonies were seen with Salmonella, Acinetobacter, Burkholderia, and Yersinia enterocolitica (turns pink). Our study suggests that Chromatic MH for dDD may show promise as a rapid, clinically useful presumptive method for overnight simultaneous identification and antimicrobial susceptibility testing. However, there is a need to optimize the medium formulation to allow the recovery of Streptococcus pneumoniae and Haemophilus influenzae.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Hemocultura / Anti-Infecciosos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2022 Tipo de documento: Article País de afiliação: África do Sul

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Hemocultura / Anti-Infecciosos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2022 Tipo de documento: Article País de afiliação: África do Sul