Multiplication of defective Ebola virus in a complementary permissive cell line.
Antiviral Res
; 209: 105491, 2023 01.
Article
em En
| MEDLINE
| ID: mdl-36526073
ABSTRACT
In an effort to develop safe and innovative in vitro models for Ebola virus (EBOV) research, we generated a recombinant Ebola virus where the glycoprotein (GP) gene was substituted with the Cre recombinase (Cre) gene by reverse genetics. This defective virus could multiply itself in a complementary permissive cell line, which could express GP and reporter protein upon exogenous Cre existence. The main features of this novel model for Ebola virus are intact viral life cycle, robust virus multiplication and normal virions morphology. The design of this model ensures its safety, excellent stability and maneuverability as a tool for virology research as well as for antiviral agent screening and drug discovery, and such a design could be further adapted to other viruses.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Doença pelo Vírus Ebola
/
Ebolavirus
Limite:
Humans
Idioma:
En
Revista:
Antiviral Res
Ano de publicação:
2023
Tipo de documento:
Article