Detection of Mycoplasma spp. in horses with respiratory disorders.
Equine Vet J
; 55(5): 747-754, 2023 Sep.
Article
em En
| MEDLINE
| ID: mdl-36572918
ABSTRACT
BACKGROUND:
Bacteria belonging to the genus Mycoplasma are small-sized, have no cell walls and small genomes. They commonly cause respiratory disorders in their animal hosts. Three species have been found in the respiratory tract of horses worldwide, that is., Mycoplasma (M.) equirhinis, M. pulmonis and M. felis, but their role in clinical cases remains unclear.OBJECTIVES:
The aim of this study was to i) develop and validate tools to detect, isolate and identify different Mycoplasma spp. strains in clinical equine respiratory-tract specimens and ii) subsequently define the prevalence of the three species in France depending on sample types and horse characteristics (age, breed, sex). STUDYDESIGN:
Validation of a workflow for mycoplasma diagnosis and subsequent prevalence study.METHODS:
Mycoplasma-free tracheal wash samples spiked with numerated strains and DNA dilutions were used to validate the culture methods and real-time PCR (rt-PCR) assay. Isolated strains were identified by 16S rRNA gene sequencing. Prevalences were determined on a population of 616 horses with respiratory disorders, sampled in France in 2020.RESULTS:
In total, 104 horses (16.9%) were found to be positive for Mycoplasma spp. by at least one method. M. equirhinis was the predominant circulating species, accounting for 85% of the rt-PCR-positive samples and 98% of the 40 cultured strains. MAINLIMITATION:
The proposed pre-enrichment procedure improves the sensitivity of detection but hinders the quantification of the initial mycoplasma load in the clinical specimens.CONCLUSIONS:
Prevalence of mycoplasma varied with age, breed, and type of sample.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Doenças Respiratórias
/
Mycoplasma
/
Infecções por Mycoplasma
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Equine Vet J
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
França