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Comprehensive characterization of fetal and mature retinal cell identity to assess the fidelity of retinal organoids.
Kim, Hani Jieun; O'Hara-Wright, Michelle; Kim, Daniel; Loi, To Ha; Lim, Benjamin Y; Jamieson, Robyn V; Gonzalez-Cordero, Anai; Yang, Pengyi.
Afiliação
  • Kim HJ; Computational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Mathematics and Statistics, The University of Sydney, Sydney, NSW 2006, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University
  • O'Hara-Wright M; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Stem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia.
  • Kim D; Computational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
  • Loi TH; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Eye Genetics Research Unit, Children's Medical Research Institute, Sydney Children's Hospitals Network, Save Sight Institute, The University of Sydney, Westmead, NSW 2145, Australia
  • Lim BY; Stem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia.
  • Jamieson RV; Specialty of Genomic Medicine, Faculty of Medicine and Health, University of Sydney, Westmead, NSW 2145, Australia; Eye Genetics Research Unit, Children's Medical Research Institute, Sydney Children's Hospitals Network, Save Sight Institute, The University of Sydney, Westmead, NSW 2145, Australia.
  • Gonzalez-Cordero A; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia; Stem Cell Medicine Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia. Electronic address: agonzalez-cordero@cmri.org.au.
  • Yang P; Computational Systems Biology Group, Children's Medical Research Institute, The University of Sydney, Westmead, NSW 2145, Australia; School of Mathematics and Statistics, The University of Sydney, Sydney, NSW 2006, Australia; School of Medical Sciences, Faculty of Medicine and Health, The University
Stem Cell Reports ; 18(1): 175-189, 2023 01 10.
Article em En | MEDLINE | ID: mdl-36630901
Characterizing cell identity in complex tissues such as the human retina is essential for studying its development and disease. While retinal organoids derived from pluripotent stem cells have been widely used to model development and disease of the human retina, there is a lack of studies that have systematically evaluated the molecular and cellular fidelity of the organoids derived from various culture protocols in recapitulating their in vivo counterpart. To this end, we performed an extensive meta-atlas characterization of cellular identities of the human eye, covering a wide range of developmental stages. The resulting map uncovered previously unknown biomarkers of major retinal cell types and those associated with cell-type-specific maturation. Using our retinal-cell-identity map from the fetal and adult tissues, we systematically assessed the fidelity of the retinal organoids in mimicking the human eye, enabling us to comprehensively benchmark the current protocols for retinal organoid generation.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Pluripotentes Induzidas Limite: Adult / Humans Idioma: En Revista: Stem Cell Reports Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Células-Tronco Pluripotentes / Células-Tronco Pluripotentes Induzidas Limite: Adult / Humans Idioma: En Revista: Stem Cell Reports Ano de publicação: 2023 Tipo de documento: Article