An intrasplenic injection model of pancreatic cancer metastasis to the liver in mice.
STAR Protoc
; 4(1): 102021, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36638017
ABSTRACT
Here, we provide a protocol for an intrasplenic injection model to establish pancreatic tumors in the mouse liver. We describe the steps to inject tumor cells into mouse spleen and to perform a splenectomy, followed by animal recovery and end point analysis of tumors in the liver. This model allows rapid and reproducible tumor growth in a clinically relevant metastatic site, providing a platform to evaluate the efficacy of anti-cancer drugs. This technique can be expanded to other cancer cell lines. For complete details on the use and execution of this protocol, please refer to Poh et al. (2022).1.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Neoplasias Hepáticas
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Austrália