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A cellular taxonomy of the adult human spinal cord.
Yadav, Archana; Matson, Kaya J E; Li, Li; Hua, Isabelle; Petrescu, Joana; Kang, Kristy; Alkaslasi, Mor R; Lee, Dylan I; Hasan, Saadia; Galuta, Ahmad; Dedek, Annemarie; Ameri, Sara; Parnell, Jessica; Alshardan, Mohammad M; Qumqumji, Feras Abbas; Alhamad, Saud M; Wang, Alick Pingbei; Poulen, Gaetan; Lonjon, Nicolas; Vachiery-Lahaye, Florence; Gaur, Pallavi; Nalls, Mike A; Qi, Yue A; Maric, Dragan; Ward, Michael E; Hildebrand, Michael E; Mery, Pierre-Francois; Bourinet, Emmanuel; Bauchet, Luc; Tsai, Eve C; Phatnani, Hemali; Le Pichon, Claire E; Menon, Vilas; Levine, Ariel J.
Afiliação
  • Yadav A; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA.
  • Matson KJE; Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA; Johns Hopkins University Department of Biology, Baltimore, MD 21218, USA.
  • Li L; Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Hua I; Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Petrescu J; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA; Center for Genomics of Neurodegenerative Disease, New York Genome Center, New York, NY, USA.
  • Kang K; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA; Center for Genomics of Neurodegenerative Disease, New York Genome Center, New York, NY, USA.
  • Alkaslasi MR; Unit on the Development of Neurodegeneration, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA; Department of Neuroscience, Brown University, Providence, RI, USA.
  • Lee DI; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA.
  • Hasan S; Inherited Neurodegenerative Diseases Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Galuta A; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Dedek A; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Neuroscience, Carleton University, Ottawa, ON, Canada.
  • Ameri S; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Parnell J; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada; Department of Neuroscience, Carleton University, Ottawa, ON, Canada.
  • Alshardan MM; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Qumqumji FA; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Alhamad SM; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Wang AP; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Poulen G; Department of Neurosurgery, Gui de Chauliac Hospital, and Donation and Transplantation Coordination Unit, Montpellier University Medical Center, Montpellier, France.
  • Lonjon N; Department of Neurosurgery, Gui de Chauliac Hospital, and Donation and Transplantation Coordination Unit, Montpellier University Medical Center, Montpellier, France.
  • Vachiery-Lahaye F; Department of Neurosurgery, Gui de Chauliac Hospital, and Donation and Transplantation Coordination Unit, Montpellier University Medical Center, Montpellier, France.
  • Gaur P; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA.
  • Nalls MA; Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, USA; Center for Alzheimer's and Related Dementias, National Institutes of Health, Bethesda, MD, USA; Data Tecnica International LLC, Glen Echo, MD, USA.
  • Qi YA; Center for Alzheimer's and Related Dementias, National Institutes of Health, Bethesda, MD, USA.
  • Maric D; Flow and Imaging Cytometry Core Facility, National Institute of Neurological Disorders and Stroke; Bethesda, MD, USA.
  • Ward ME; Inherited Neurodegenerative Diseases Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
  • Hildebrand ME; Inherited Neurodegenerative Diseases Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Mery PF; Institute of Functional Genomics, Montpellier University, CNRS, INSERM, Montpellier, France.
  • Bourinet E; Institute of Functional Genomics, Montpellier University, CNRS, INSERM, Montpellier, France.
  • Bauchet L; Department of Neurosurgery, Gui de Chauliac Hospital, and Donation and Transplantation Coordination Unit, Montpellier University Medical Center, Montpellier, France; Institute of Functional Genomics, Montpellier University, CNRS, INSERM, Montpellier, France.
  • Tsai EC; Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada.
  • Phatnani H; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA; Center for Genomics of Neurodegenerative Disease, New York Genome Center, New York, NY, USA.
  • Le Pichon CE; Unit on the Development of Neurodegeneration, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA.
  • Menon V; Department of Neurology, Center for Translational and Computational Neuroimmunology, Columbia University, New York, NY, USA. Electronic address: vm2545@cumc.columbia.edu.
  • Levine AJ; Spinal Circuits and Plasticity Unit, National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. Electronic address: ariel.levine@nih.gov.
Neuron ; 111(3): 328-344.e7, 2023 02 01.
Article em En | MEDLINE | ID: mdl-36731429
ABSTRACT
The mammalian spinal cord functions as a community of cell types for sensory processing, autonomic control, and movement. While animal models have advanced our understanding of spinal cellular diversity, characterizing human biology directly is important to uncover specialized features of basic function and human pathology. Here, we present a cellular taxonomy of the adult human spinal cord using single-nucleus RNA sequencing with spatial transcriptomics and antibody validation. We identified 29 glial clusters and 35 neuronal clusters, organized principally by anatomical location. To demonstrate the relevance of this resource to human disease, we analyzed spinal motoneurons, which degenerate in amyotrophic lateral sclerosis (ALS) and other diseases. We found that compared with other spinal neurons, human motoneurons are defined by genes related to cell size, cytoskeletal structure, and ALS, suggesting a specialized molecular repertoire underlying their selective vulnerability. We include a web resource to facilitate further investigations into human spinal cord biology.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Esclerose Lateral Amiotrófica Limite: Adult / Animals / Humans Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Esclerose Lateral Amiotrófica Limite: Adult / Animals / Humans Idioma: En Revista: Neuron Assunto da revista: NEUROLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos