Assessment of homozygosity in transgenic plants using selectable markers.
STAR Protoc
; 4(1): 102031, 2023 03 17.
Article
em En
| MEDLINE
| ID: mdl-36853737
ABSTRACT
Production of homozygous transgenic plants is a prerequisite for the phenotypic analysis and/or for the commercial release of transgenic plants for cultivation. Here we present a simple protocol for the selection of homozygous transgenics using antibiotics as a selectable marker. The protocol has been used to select homozygous rice transgenic plants using hygromycin antibiotic. However, the described protocol can be used for selction of homozygous in any transgenic plants using a appropriate selectable marker. For complete details on the use and execution of this protocol, please refer to Passricha et al. (2016).1.
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Bases de dados:
MEDLINE
Assunto principal:
Oryza
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Índia