A step sensitive to pertussis toxin and phorbol ester in human neutrophils regulates chemotaxis and capping but not phagocytosis.
FEBS Lett
; 200(1): 91-6, 1986 May 05.
Article
em En
| MEDLINE
| ID: mdl-3699167
ABSTRACT
Treatment of human neutrophils with pertussis toxin (PT) abolishes chemotaxis in response to either platelet-activating factor (PAF) or f-Met-Leu-Phe (FMLP), and capping induced via the concanavalin A (Con A) receptor. These functional effects are accompanied by the inhibition of calcium mobilization by PAF, FMLP and Con A. The agent phorbol 12-myristate-13-acetate (PMA) also inhibits chemotaxis and capping as well as calcium mobilization by these receptors. In sharp contrast, neither PT, cholera toxin (CT), nor PMA, inhibits the phagocytosis of non-opsonized and opsonized Candida albicans, sheep erythrocytes or fluorescent latex beads. Our results suggest that receptor-initiated chemotaxis and capping involve a step that is sensitive to PT and PMA, and that phagocytosis is not regulated in a similar fashion.
Buscar no Google
Bases de dados:
MEDLINE
Assunto principal:
Fagocitose
/
Fatores de Virulência de Bordetella
/
Forbóis
/
Acetato de Tetradecanoilforbol
/
Quimiotaxia de Leucócito
/
Fluoresceína-5-Isotiocianato
/
Toxina Pertussis
/
Neutrófilos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1986
Tipo de documento:
Article