Role of periosteum during healing of alveolar critical size bone defects in the mandible: a pilot study.
Clin Oral Investig
; 27(8): 4541-4552, 2023 Aug.
Article
em En
| MEDLINE
| ID: mdl-37261496
ABSTRACT
OBJECTIVES:
Minipigs present advantages for studying oral bone regeneration; however, standardized critical size defects (CSD) for alveolar bone have not been validated yet. The objectives of this study are to develop a CSD in the mandibular alveolar bone in Aachen minipigs and to further investigate the specific role of periosteum. MATERIALS ANDMETHODS:
Three female Aachen minipigs aged 17, 24, and 84 months were used. For each minipig, a split-mouth design was performed an osteotomy (2 cm height × 2.5 cm length) was performed; the periosteum was preserved on the left side and removed on the right side. Macroscopic, cone beam computed tomography (CBCT), microcomputed tomography (µCT), and histological analyses were performed to evaluate the bone defects and bone healing.RESULTS:
In both groups, spontaneous healing was insufficient to restore initial bone volume. The macroscopic pictures and the CBCT results showed a larger bone defect without periosteum. µCT results revealed that BMD, BV/TV, and Tb.Th were significantly lower without periosteum. The histological analyses showed (i) an increased osteoid apposition in the crestal area when periosteum was removed and (ii) an ossification process in the mandibular canal area in response to the surgical that seemed to increase when periosteum was removed.CONCLUSIONS:
A robust model of CSD model was developed in the alveolar bone of minipigs that mimics human mandibular bone defects. This model allows to further investigate the bone healing process and potential factors impacting healing such as periosteum. CLINICAL RELEVANCE This model may be relevant for testing different bone reconstruction strategies for preclinical investigations.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Periósteo
/
Regeneração Óssea
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Clin Oral Investig
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
França