Multifilament Collagen Fiber Bundles with Tendon-like Structure and Mechanical Performance.
Macromol Rapid Commun
; 44(18): e2300204, 2023 Sep.
Article
em En
| MEDLINE
| ID: mdl-37291949
ABSTRACT
Collagen multifilament bundles comprised of thousands of monofilaments are prepared by multipin contact drawing of an entangled polymer solution consisting of collagen and poly(ethylene oxide) (PEO). The multifilament bundles are hydrated in graded concentrations of PEO and phosphate buffered saline (PBS) to promote assembly of collagen fibrils within each monofilament while preserving the structure of the multifilament bundle. Multiscale structural characterization reveals that the hydrated multifilament bundle contains properly folded collagen molecules packed in collagen fibrils containing microfibrils, staggered by exactly one-sixth of the microfibril D-band spacing to produce a periodicity of 11 nm. Sequence analysis predicts that in this structure, phenylalanine residues are close enough within and between microfibrils to become ultraviolet C (UVC) crosslinked. In agreement with this analysis, the ultimate tensile strength (UTS) and Young's modulus of the hydrated collagen multifilament bundles crosslinked by UVC radiation increase nonlinearly with total UVC energy to reach values in the range of native tendons without damage to the collagen molecules. This fabrication method recapitulates the structure of a tendon across multiple length scales and offers tunability in tensile properties using only collagen molecules and no other chemical additives in addition to PEO, which is almost entirely removed during the hydration process.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Tendões
/
Colágeno
Idioma:
En
Revista:
Macromol Rapid Commun
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Canadá