Metabolism of benzo(a)pyrene in monolayer cultures of human bronchial epithelial cells from a series of donors.
Cancer Res
; 46(9): 4368-71, 1986 Sep.
Article
em En
| MEDLINE
| ID: mdl-3731094
ABSTRACT
Benzo(a)pyrene [B(a)P] metabolism was measured in monolayer cultures of human bronchial epithelial cells derived from 18 specimens of explanted tissue. Bronchial epithelial cells converted B(a)P to dihydrodiols, phenols, quinone derivatives, and polyhydroxylated forms. Sulfate and glucuronide conjugates of B(a)P metabolites were also detected. Both total metabolism and distribution of metabolites showed a 10-fold or greater variation in cultures from different specimens. When the data were divided according to smoking status, however, no differences in total metabolism, extent of conjugation, or distribution of metabolites could be demonstrated between the two groups. Wide variation (over 1000-fold) in the cytotoxicity of B(a)P towards cells derived from different specimens was demonstrated but could not be directly correlated to the extent of metabolic activation. The results suggest that human bronchial epithelial cells which are newly grown from explanted tissue of smokers in culture do not demonstrate enzymatic induction. Variation among individuals observed in these studies probably represents basal differences in metabolic capability.
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Bases de dados:
MEDLINE
Assunto principal:
Benzo(a)pireno
/
Brônquios
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1986
Tipo de documento:
Article