High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution.
bioRxiv
; 2023 Jun 06.
Article
em En
| MEDLINE
| ID: mdl-37333143
ABSTRACT
Brain research is an area of research characterized by its cutting-edge nature, with brain mapping constituting a crucial aspect of this field. As sequencing tools have played a crucial role in gene sequencing, brain mapping largely depends on automated, high-throughput and high-resolution imaging techniques. Over the years, the demand for high-throughput imaging has scaled exponentially with the rapid development of microscopic brain mapping. In this paper, we introduce the novel concept of confocal Airy beam into oblique light-sheet tomography named CAB-OLST. We demonstrate that this technique enables the high throughput of brain-wide imaging of long-distance axon projection for the entire mouse brain at a resolution of 0.26 µm × 0.26 µm × 1.06 µm in 58 hours. This technique represents an innovative contribution to the field of brain research by setting a new standard for high-throughput imaging techniques.
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MEDLINE
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En
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BioRxiv
Ano de publicação:
2023
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Article