Study of Agonists of TLRs as Vaccine Adjuvants.
Methods Mol Biol
; 2700: 249-269, 2023.
Article
em En
| MEDLINE
| ID: mdl-37603186
ABSTRACT
Vaccines adjuvants are critically needed to enhance the effectiveness of subunit vaccines. Due to their ability to link the innate with the adaptive immune response, Toll-like receptor (TLR) agonists have received great attention as adjuvants in vaccines against severe and complex diseases such as cancer, AIDS, and malaria. Here, we describe in vitro assays, e.g., the Monocyte Activation Test, TLR-specific activation assay, and TLR-blocking experiments, used to assess TLR agonists adjuvanted vaccines' safety and to characterize their ability to stimulate the innate immunity. Such assays are physiologically relevant as they work with human cells and allow to overcome the complexity and variability related to in vivo assays.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Adjuvantes Imunológicos
/
Adjuvantes de Vacinas
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Itália