Anammox granule destruction and reconstruction in a partial nitrification/anammox system under hydroxylamine stress.
J Environ Manage
; 345: 118688, 2023 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-37660422
ABSTRACT
Nitrite oxidizing bacteria (NOB) outcompeting anammox bacteria (AnAOB) poses a challenge to the practical implementation of the partial nitrification/anammox (PN/A) process for municipal wastewater. A granules-based PN/A bioreactor was operated for 260 d with hydroxylamine (NH2OH) added halfway through. qPCR results detected the different amounts of NOB among granules and flocs and the dynamic succession during operation. CLSM images revealed a unique layered structure of granules that NOB located inside led to the inhibition effect of NH2OH delayed. Besides, the physical and morphological characteristics revealed that anammox granules experienced destruction. AnAOB took the broken granules as an initial biofilm aggregate to reconstruct new granules. RT-qPCR and high throughput sequencing results suggested that functional gene expression and community structure were regulated for the AnAOB metabolism process. Correspondingly, the rapid proliferation (0.52 â 1.99%) of AnAOB was realized, and the nitrogen removal rate achieved a nearly quadruple improvement (0.21 â 0.83 kg-N/m3·d). This study revealed that anammox granules can self-reconstruct in the PN/A system when granules are disintegrated under NH2OH stress, broadening the feasibility of applying PN/A process.
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Texto completo:
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Bases de dados:
MEDLINE
Assunto principal:
Nitrificação
/
Oxidação Anaeróbia da Amônia
Idioma:
En
Revista:
J Environ Manage
Ano de publicação:
2023
Tipo de documento:
Article