Rational design of a ratiometric fluorescent nanoprobe for real-time imaging of hydroxyl radical and its therapeutic evaluation of diabetes.
Biosens Bioelectron
; 246: 115868, 2024 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-38029709
ABSTRACT
Hydroxyl radical (â¢OH), one of the most reactive and deleterious substances in organisms, belongs to a class of reactive oxygen species (ROS), and it has been verified to play an essential role in numerous pathophysiological scenarios. However, due to its extremely high reactivity and short lifetime, the development of a reliable and robust method for tracking endogenous â¢OH remains an ongoing challenge. In this work, we presented the first ratiometric fluorescent nanoprobe NanoDCQ-3 for â¢OH sensing based on oxidative C-H abstraction of dihydroquinoline to quinoline. The study mainly focused on how to modulate the electronic effects to achieve an ideal ratiometric detection of â¢OH, as well as solving the inherent problem of hydrophilicity of the probe, so that it was more conducive to monitoring â¢OH in living organisms. The screened-out probe NanoDCQ-3 exhibited an exceptional ratiometric sensing capability, better biocompatibility, good cellular uptake, and appropriate in vivo retention, which has been reliably used for detecting exogenous â¢OH concentration fluctuation in living cells and zebrafish models. More importantly, NanoDCQ-3 facilitated visualization of â¢OH and evaluation of drug treatment efficacy in diabetic mice. These findings afforded a promising strategy for designing ratiometric fluorescent probes for â¢OH. NanoDCQ-3 emerged as a valuable tool for the detection of â¢OH in vivo and held potential for drug screening for inflammation-related diseases.
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Bases de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Diabetes Mellitus Experimental
Limite:
Animals
Idioma:
En
Revista:
Biosens Bioelectron
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China