Evaluation of five multisteroid LCâMS/MS methods used for routine clinical analysis: comparable performance was obtained for nine analytes.
Clin Chem Lab Med
; 62(5): 900-910, 2024 Apr 25.
Article
em En
| MEDLINE
| ID: mdl-38038605
ABSTRACT
OBJECTIVES:
A mass spectrometry (LCâMS/MS)-based interlaboratory comparison study was performed for nine steroid analytes with five participating laboratories. The sample set contained 40 pooled samples of human serum generated from preanalyzed leftovers. To obtain a well-balanced distribution across reference intervals of each steroid, the leftovers first underwent a targeted mixing step.METHODS:
All participants measured a sample set once using their own multianalyte protocols and calibrators. Four participants used in-house developed measurement platforms, including IVD-CE certified calibrators, which were used by three participants; the 5th lab used the whole LCâMS kit from an IVD manufacturer. All labs reported results for 17-hydroxyprogesterone, androstenedione, cortisol, and testosterone, and four labs reported results for 11-deoxycortisol, corticosterone, cortisone, dehydroepiandrosterone sulfate (DHEAS), and progesterone.RESULTS:
Good or acceptable overall comparability was found in BlandâAltman and PassingâBablok analyses. Mean bias against the overall mean remained less than ±10â¯% except for DHEAS, androstenedione, and progesterone at one site and for cortisol and corticosterone at two sites (max. -18.9â¯% for androstenedione). The main analytical problems unraveled by this study included a bias not previously identified in proficiency testing, operator errors, non-supported matrix types and higher inaccuracy and imprecision at lower ends of measuring intervals.CONCLUSIONS:
This study shows that intermethod comparison is essential for monitoring the validity of an assay and should serve as an example of how external quality assessment could work in addition to organized proficiency testing schemes.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Progesterona
/
Hidrocortisona
Limite:
Humans
Idioma:
En
Revista:
Clin Chem Lab Med
Assunto da revista:
QUIMICA CLINICA
/
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Áustria