Quantification of growth and nutrient consumption of bacterial and fungal cultures in microfluidic microhabitat models.
STAR Protoc
; 5(1): 102784, 2024 Mar 15.
Article
em En
| MEDLINE
| ID: mdl-38103191
ABSTRACT
Understanding microbes in nature requires consideration of their microenvironment. Here, we present a protocol for quantifying biomass and nutrient degradation of bacterial and fungal cultures (Pseudomonas putida and Coprinopsis cinerea, respectively) in microfluidics. We describe steps for mask design and fabrication, master printing, polydimethylsiloxane chip fabrication, and chip inoculation and imaging using fluorescence microscopy. We include procedures for image analysis, plotting, and statistics. For complete details on the use and execution of this protocol, please refer to Arellano-Caicedo et al. (2023).1.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Processamento de Imagem Assistida por Computador
/
Microfluídica
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article