Molecular characterization and allergenicity assessment of different samples of Mung Bean.
Food Chem X
; 20: 100980, 2023 Dec 30.
Article
em En
| MEDLINE
| ID: mdl-38144835
ABSTRACT
Legumes represent a promising nutritional alternative source of proteins to meat and dairy products. Additionally, Novel Foods (Regulation EU 2015/2283) can help meet the rising protein demand. However, despite their benefits, emerging allergenicity risks must be considered. The aim of this work is the molecular characterization of the Novel Food Mung bean protein isolate for allergenicity prediction with High Resolution Mass Spectrometry analysis. The assessment of the allergenicity was evaluated in silico by comparing protein sequences of the Novel Food with other known legume allergens, using bioinformatic databases. The results highlighted similarity higher than 60 % of the protein structure of Mung bean with two known allergens of soybean and pea. Furthermore, enzymatic hydrolysis effects on allergenic potential was evaluated by immunoblotting analysis using sera of patients allergic to legumes. The protein hydrolysates obtained showed a high nutritional quality and a reduced allergenic potential, making them suitable for hypoallergenic food formulations.
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Bases de dados:
MEDLINE
Idioma:
En
Revista:
Food Chem X
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Itália