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Microglia enhance post-anesthesia neuronal activity by shielding inhibitory synapses.
Haruwaka, Koichiro; Ying, Yanlu; Liang, Yue; Umpierre, Anthony D; Yi, Min-Hee; Kremen, Vaclav; Chen, Tingjun; Xie, Tao; Qi, Fangfang; Zhao, Shunyi; Zheng, Jiaying; Liu, Yong U; Dong, Hailong; Worrell, Gregory A; Wu, Long-Jun.
Afiliação
  • Haruwaka K; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Ying Y; Department of Anesthesiology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China. eyyingyanlu@scut.edu.cn.
  • Liang Y; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Umpierre AD; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Yi MH; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Kremen V; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Chen T; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Xie T; Translational Oncology Group, Faculty of Science, School of Life Sciences, University of Technology Sydney, Sydney, New South Wales, Australia.
  • Qi F; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Zhao S; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Zheng J; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Liu YU; Department of Anesthesiology, Guangzhou First People's Hospital, South China University of Technology, Guangzhou, China.
  • Dong H; Department of Anesthesiology and Perioperative Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
  • Worrell GA; Department of Neurology, Mayo Clinic, Rochester, MN, USA.
  • Wu LJ; Department of Neurology, Mayo Clinic, Rochester, MN, USA. wu.longjun@mayo.edu.
Nat Neurosci ; 27(3): 449-461, 2024 Mar.
Article em En | MEDLINE | ID: mdl-38177340
ABSTRACT
Microglia are resident immune cells of the central nervous system and play key roles in brain homeostasis. During anesthesia, microglia increase their dynamic process surveillance and interact more closely with neurons. However, the functional significance of microglial process dynamics and neuronal interaction under anesthesia is largely unknown. Using in vivo two-photon imaging in mice, we show that microglia enhance neuronal activity after the cessation of isoflurane anesthesia. Hyperactive neuron somata are contacted directly by microglial processes, which specifically colocalize with GABAergic boutons. Electron-microscopy-based synaptic reconstruction after two-photon imaging reveals that, during anesthesia, microglial processes enter into the synaptic cleft to shield GABAergic inputs. Microglial ablation or loss of microglial ß2-adrenergic receptors prevents post-anesthesia neuronal hyperactivity. Our study demonstrates a previously unappreciated function of microglial process dynamics, which enable microglia to transiently boost post-anesthesia neuronal activity by physically shielding inhibitory inputs.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Microglia / Anestesia Limite: Animals Idioma: En Revista: Nat Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Microglia / Anestesia Limite: Animals Idioma: En Revista: Nat Neurosci Assunto da revista: NEUROLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos