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A simple and sensitive liquid chromatography triple quadrupole mass spectrometry method for the determination of XL092 in monkey plasma and its application to pharmacokinetic study.
Li, Cui; Li, Xiaokun; Fan, Ali; He, Ning; Wu, Dongmei; Yu, Hongyan; Wang, Kun; Jiao, Weijie; Zhao, Xu.
Afiliação
  • Li C; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Li X; Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Fan A; TriApex Laboratories Co. Ltd, Nanjing, China.
  • He N; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Wu D; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Yu H; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Wang K; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Jiao W; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
  • Zhao X; Department of Pharmacy, Henan Province Hospital of Traditional Chinese Medicine, The Second Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, Henan Province, China.
Biomed Chromatogr ; 38(5): e5833, 2024 May.
Article em En | MEDLINE | ID: mdl-38291606
ABSTRACT
XL092 is a potent ATP-competitive inhibitor of multiple receptor tyrosine kinases that is undergoing clinical development for the treatment of lung cancer. In this study, an LC triple quadrupole mass spectrometry method was established to measure XL092 in monkey plasma. A Waters ACQUITY UPLC BEH C18 column was used for chromatographic separation. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile with a gradient elution at the flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of XL092 in positive electrospray ionization. In the concentration range of 0.5-1000 ng/mL, XL092 showed excellent linearity in monkey plasma with a correlation coefficient greater than 0.995 (r > 0.995). The lowest limit of quantification was 0.5 ng/mL. The intra- and inter-day relative standard deviations were <9.99%, while the relative error ranged from -12.50% to 8.10%. The mean recovery was over 82.51%. XL092 was stable in monkey plasma after storage under certain conditions. The validated method was demonstrated to be selective, sensitive, and reliable, and has been successfully applied to the pharmacokinetic study of XL092 in monkey plasma. XL092 showed moderate short half-life (~3.81 h) and good oral bioavailability (80%).
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Biomed Chromatogr Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Espectrometria de Massas em Tandem Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Biomed Chromatogr Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China