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A new bio imagery user-friendly tool for automatic morphometry measurement on muscle cell cultures and histological sections.
Brun, Aurélien; Mougeot, Guillaume; Denis, Philippe; Collin, Marie Laure; Pouchin, Pierre; Montaurier, Christophe; Walrand, Stéphane; Capel, Frédéric; Gueugneau, Marine.
Afiliação
  • Brun A; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
  • Mougeot G; iGReD CNRS, INSERM Université Clermont Auvergne, Clermont-Ferrand, France.
  • Denis P; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
  • Collin ML; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
  • Pouchin P; iGReD CNRS, INSERM Université Clermont Auvergne, Clermont-Ferrand, France.
  • Montaurier C; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
  • Walrand S; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
  • Capel F; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France. frederic.capel@inrae.fr.
  • Gueugneau M; UMR1019 Unité de Nutrition Humaine (UNH), INRAE, Université Clermont Auvergne, Clermont-Ferrand, France.
Sci Rep ; 14(1): 3108, 2024 02 07.
Article em En | MEDLINE | ID: mdl-38326394
ABSTRACT
TRUEFAD (TRUE Fiber Atrophy Distinction) is a bioimagery user-friendly tool developed to allow consistent and automatic measurement of myotube diameter in vitro, muscle fiber size and type using rodents and human muscle biopsies. This TRUEFAD package was set up to standardize and dynamize muscle research via easy-to-obtain images run on an open-source plugin for FIJI. We showed here both the robustness and the performance of our pipelines to correctly segment muscle cells and fibers. We evaluated our pipeline on real experiment image sets and showed consistent reliability across images and conditions. TRUEFAD development makes possible systematical and rapid screening of substances impacting muscle morphology for helping scientists focus on their hypothesis rather than image analysis.
Assuntos

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Software / Fibras Musculares Esqueléticas Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Software / Fibras Musculares Esqueléticas Limite: Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França