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Infrared photoinduced electrochemiluminescence microscopy of single cells.
Descamps, Julie; Zhao, Yiran; Goudeau, Bertrand; Manojlovic, Dragan; Loget, Gabriel; Sojic, Neso.
Afiliação
  • Descamps J; Univ. Bordeaux, CNRS UMR 5255, Bordeaux INP, Site ENSMAC 33607 Pessac France sojic@u-bordeaux.fr.
  • Zhao Y; Univ. Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR6226 Rennes F-35000 France gabriel.loget@cnrs.fr.
  • Goudeau B; Univ. Bordeaux, CNRS UMR 5255, Bordeaux INP, Site ENSMAC 33607 Pessac France sojic@u-bordeaux.fr.
  • Manojlovic D; University of Belgrade, Faculty of Chemistry 11000 Belgrade Serbia.
  • Loget G; Univ. Rennes, CNRS, ISCR (Institut des Sciences Chimiques de Rennes)-UMR6226 Rennes F-35000 France gabriel.loget@cnrs.fr.
  • Sojic N; Institute of Energy and Climate Research, Fundamental Electrochemistry (IEK-9), Forschungszentrum Jülich GmbH Jülich 52425 Germany.
Chem Sci ; 15(6): 2055-2061, 2024 Feb 07.
Article em En | MEDLINE | ID: mdl-38332811
ABSTRACT
Electrochemiluminescence (ECL) is evolving rapidly from a purely analytical technique into a powerful microscopy. Herein, we report the imaging of single cells by photoinduced ECL (PECL; λem = 620 nm) stimulated by an incident near-infrared light (λexc = 1050 nm). The cells were grown on a metal-insulator-semiconductor (MIS) n-Si/SiOx/Ir photoanode that exhibited stable and bright PECL emission. The large anti-Stokes shift allowed for the recording of well-resolved images of cells with high sensitivity. PECL microscopy is demonstrated at a remarkably low onset potential of 0.8 V; this contrasts with classic ECL, which is blind at this potential. Two imaging modes are reported (i) photoinduced positive ECL (PECL+), showing the cell membranes labeled with the [Ru(bpy)3]2+ complex; and (ii) photoinduced shadow label-free ECL (PECL-) of cell morphology, with the luminophore in the solution. Finally, by adding a new dimension with the near-infrared light stimulus, PECL microscopy should find promising applications to image and study single photoactive nanoparticles and biological entities.

Texto completo: 1 Bases de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Chem Sci Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Tipo de estudo: Clinical_trials Idioma: En Revista: Chem Sci Ano de publicação: 2024 Tipo de documento: Article