A multifaceted investigation of lactococcal strain diversity in undefined mesophilic starter cultures.
Appl Environ Microbiol
; 90(3): e0215223, 2024 Mar 20.
Article
em En
| MEDLINE
| ID: mdl-38334291
ABSTRACT
The dairy fermentation industry relies on the activity of lactic acid bacteria in robust starter cultures to accomplish milk acidification. Maintenance of the composition of these starter cultures, whether defined or undefined, is essential to ensure consistent and high-quality fermentation end products. To date, limited information exists regarding the microbial composition of undefined starter culture systems. Here, we describe a culture-based analysis combined with a metagenomics approach to evaluate the composition of two undefined mesophilic starter cultures. In addition, we describe a qPCR-based genotype detection assay, which is capable of discerning nine distinct lactococcal genotypes to characterize these undefined starter cultures, and which can be applied to monitor compositional changes in an undefined starter culture during a fermentation. IMPORTANCE This study reports on the development of a combined culture-based analysis and metagenomics approach to evaluate the composition of two undefined mesophilic starter cultures. In addition, a novel qPCR-based genotype detection assay, capable of discerning nine distinct lactococcal genotypes (based on lactococcal cell wall polysaccharide biosynthesis gene clusters), was used to monitor compositional changes in an undefined starter culture following phage attack. These analytical approaches facilitate a multifaceted assessment of starter culture compositional stability during milk fermentation, which has become an important QC aspect due to the increasing demand for consistent and high-quality dairy products.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Bacteriófagos
/
Lactococcus lactis
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Lactobacillales
Limite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Irlanda