Far-red chemigenetic biosensors for multi-dimensional and super-resolved kinase activity imaging.

Frei, Michelle S; Sanchez, Samantha A; Liu, Longwei; Schneider, Falk; Wang, Zichen; Hakozaki, Hiroyuki; Li, Yajuan; Lyons, Anne C; Rohm, Theresa V; Olefsky, Jerrold M; Shi, Lingyan; Schöneberg, Johannes; Fraser, Scott E; Mehta, Sohum; Wang, Yingxiao; Zhang, Jin

Frei, Michelle S; Sanchez, Samantha A; Liu, Longwei; Schneider, Falk; Wang, Zichen; Hakozaki, Hiroyuki; Li, Yajuan; Lyons, Anne C; Rohm, Theresa V; Olefsky, Jerrold M; Shi, Lingyan; Schöneberg, Johannes; Fraser, Scott E; Mehta, Sohum; Wang, Yingxiao; Zhang, Jin.

Afiliação

Frei MS; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Sanchez SA; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Liu L; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Schneider F; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Wang Z; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
Hakozaki H; Alfred E. Mann Department of Biomedical Engineering, University of Southern California, Los Angeles, CA, USA.
Li Y; Translational Imaging Center, University of Southern California, Los Angeles, CA, USA.
Lyons AC; Dana and David Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA, USA.
Rohm TV; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Olefsky JM; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
Shi L; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Schöneberg J; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, USA.
Fraser SE; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Mehta S; Department of Pharmacology, University of California, San Diego, La Jolla, CA, USA.
Wang Y; Shu Chien-Gene Lay Department of Bioengineering, University of California, San Diego, La Jolla, CA, USA.
Zhang J; Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla, CA, USA.

bioRxiv ; 2024 Feb 11.

Article em En | MEDLINE | ID: mdl-38370804

ABSTRACT

ABSTRACT

Fluorescent biosensors revolutionized biomedical science by enabling the direct measurement of signaling activities in living cells, yet the current technology is limited in resolution and dimensionality. Here, we introduce highly sensitive chemigenetic kinase activity biosensors that combine the genetically encodable self-labeling protein tag HaloTag7 with bright far-red-emitting synthetic fluorophores. This technology enables five-color biosensor multiplexing, 4D activity imaging, and functional super-resolution imaging via stimulated emission depletion (STED) microscopy.

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Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos