Melatonin restores DNFB-induced dysbiosis of skin microbiota in a mouse model of atopic dermatitis.
Life Sci
; 342: 122513, 2024 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-38387700
ABSTRACT
BACKGROUND:
The epidermic microbiota plays crucial roles in the pathogenesis of atopic dermatitis (AD), a common inflammatory skin disease. Melatonin (MLT) has been shown to ameliorate skin damage in AD patients, yet the underlying mechanism is unclear.METHODS:
Using 2,4-dinitrofluorobenzene (DNFB) to induce an AD model, MLT intervention was applied for 14 days to observe its pharmaceutical effect. Skin lesions were observed using HE staining, toluidine blue staining and electron microscopy. Dermal proinflammatory factor (IL-4 and IL-13) and intestinal barrier indices (ZO1 and Occludin) were assessed by immunohistochemistry and RT-qPCR, respectively. The dysbiotic microbiota was analyzed using 16S rRNA sequencing.RESULTS:
MLT significantly improved skin lesion size; inflammatory status (mast cells, IgE, IL-4, and IL-13); and the imbalance of the epidermal microbiota in AD mice. Notably, Staphylococcus aureus is the key bacterium associated with dysbiosis of the epidermal microbiota and may be involved in the fine modulation of mast cells, IL-4, IL-13 and IgE. Correlation analysis between AD and the gut revealed that intestinal dysbiosis occurred earlier than that of the pathological structure in the gut.CONCLUSION:
Melatonin reverses DNFB-induced skin damage and epidermal dysbiosis, especially in S. aureus.Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Dermatopatias
/
Dermatite Atópica
/
Microbiota
/
Melatonina
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Life Sci
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China