Developmental validation of the STRSeqTyper122 kit for massively parallel sequencing of forensic STRs.
Int J Legal Med
; 138(4): 1255-1264, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38416217
ABSTRACT
Massively parallel sequencing allows for integrated genotyping of different types of forensic markers, which reduces DNA consumption, simplifies experimental processes, and provides additional sequence-based genetic information. The STRseqTyper122 kit genotypes 63 autosomal STRs, 16 X-STRs, 42 Y-STRs, and the Amelogenin locus. Amplicon sizes of 117 loci were below 300 bp. In this study, MiSeq FGx sequencing metrics for STRseqTyper122 were presented. The genotyping accuracy of this kit was examined by comparing to certified genotypes of NIST standard reference materials and results from five capillary electrophoresis-based kits. The sensitivity of STRseqTyper122 reached 125 pg, and > 80% of the loci were correctly called with 62.5 pg and 31.25 pg input genomic DNA. Repeatability, species specificity, and tolerance for DNA degradation and PCR inhibitors of this kit were also evaluated. STRseqTyper122 demonstrated reliable performance with routine case-work samples and provided a powerful tool for forensic applications.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Impressões Digitais de DNA
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Repetições de Microssatélites
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Sequenciamento de Nucleotídeos em Larga Escala
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Int J Legal Med
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Int. j. legal med
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International journal of legal medicine
Assunto da revista:
JURISPRUDENCIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China