Your browser doesn't support javascript.
loading
Oroxin A from Oroxylum indicum improves disordered lipid metabolism by inhibiting SREBPs in oleic acid-induced HepG2 cells and high-fat diet-fed non-insulin-resistant rats.
Cai, Tianqi; Xu, Xiaoxue; Dong, Ling; Liang, Shufei; Xin, Meiling; Wang, Tianqi; Li, Tianxing; Wang, Xudong; Zheng, Weilong; Wang, Chao; Xu, Zhengbao; Wang, Meng; Song, Xinhua; Li, Lingru; Li, Jingda; Sun, Wenlong.
Afiliação
  • Cai T; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Xu X; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Dong L; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Liang S; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Xin M; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Wang T; College of Life Science, Yangtze University, Jingzhou, Hubei, 434000, People's Republic of China.
  • Li T; National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100000, People's Republic of China.
  • Wang X; College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, Zhejiang 310000, People's Republic of China.
  • Zheng W; Institute of Biomass Resources, Taizhou University, Taizhou, Zhejiang, 317700, People's Republic of China.
  • Wang C; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Xu Z; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Wang M; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Song X; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
  • Li L; National Institute of TCM Constitution and Preventive Medicine, Beijing University of Chinese Medicine, Beijing, 100000, People's Republic of China.
  • Li J; College of Life Science, Yangtze University, Jingzhou, Hubei, 434000, People's Republic of China.
  • Sun W; School of Life Sciences and Medicine, Shandong University of Technology, Zibo, Shandong, 255000, People's Republic of China.
Heliyon ; 10(7): e29168, 2024 Apr 15.
Article em En | MEDLINE | ID: mdl-38617966
ABSTRACT

Background:

Lipid metabolism disorders have become a major global public health issue. Due to the complexity of these diseases, additional research and drugs are needed. Oroxin A, the major component of Oroxylum indicum (L.) Kurz (Bignoniaceae), can improve the lipid profiles of diabetic and insulin-resistant (IR) rats. Because insulin resistance is strongly correlated with lipid metabolism, improving insulin resistance may also constitute an effective strategy for improving lipid metabolism. Thus, additional research on the efficacy and mechanism of oroxin An under non-IR conditions is needed.

Methods:

In this study, we established lipid metabolism disorder model rats by high-fat diet feeding and fatty HepG2 cell lines by treatment with oleic acid and evaluated the therapeutic effect and mechanism of oroxin A in vitro and in vivo through biochemical indicator analysis, pathological staining, immunoblotting, and immunofluorescence staining.

Results:

Oroxin A improved disordered lipid metabolism under non-IR conditions, improved the plasma and hepatic lipid profiles, and enhanced the lipid-lowering action of atorvastatin. Additionally, oroxin A reduced the total triglyceride (TG) levels by inhibiting sterol regulatory element-binding protein 1 (SREBP1) expression and reducing the expression of acetyl coenzyme A carboxylase (ACC) and fatty acid synthase (FASN) in vivo and in vitro. Oroxin A also reduced the total cholesterol (TC) levels by inhibiting SREBP2 expression and reducing HMGCR expression in vivo and in vitro. In addition, oroxin A bound to low-density lipoprotein receptor (LDLR) and increased AMPK phosphorylation.

Conclusions:

Our results suggested that oroxin A may modulate the nuclear transcriptional activity of SREBPs by binding to LDLR proteins and increasing AMPK phosphorylation. Oroxin A may thus reduce lipid synthesis and could be used for the treatment and prevention of lipid metabolism disorders.
Palavras-chave

Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2024 Tipo de documento: Article