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Barley yellow dwarf virus-GAV 17K protein disrupts thiamine biosynthesis to facilitate viral infection in plants.
Han, Xiaoyu; Yang, Xue; Chen, Siyu; Wang, He; Liu, Xiaomin; Wang, Daowen; Yang, Jin; Chen, Linlin; Sun, Bingjian; Li, Honglian; Shi, Yan.
Afiliação
  • Han X; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Yang X; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Chen S; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Wang H; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Liu X; Institute of Cereal and Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang, 050031, China.
  • Wang D; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
  • Yang J; College of Agronomy, Henan Agricultural University, Zhengzhou, 450046, China.
  • Chen L; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Sun B; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Li H; College of Plant Protection, Henan Agricultural University, Zhengzhou, 450046, China.
  • Shi Y; National Key Laboratory of Wheat and Maize Crop Science, Zhengzhou, 450046, China.
Plant J ; 119(1): 432-444, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38635415
ABSTRACT
Thiamine functions as a crucial activator modulating plant health and broad-spectrum stress tolerances. However, the role of thiamine in regulating plant virus infection is largely unknown. Here, we report that the multifunctional 17K protein encoded by barley yellow dwarf virus-GAV (BYDV-GAV) interacted with barley pyrimidine synthase (HvTHIC), a key enzyme in thiamine biosynthesis. HvTHIC was found to be localized in chloroplast via an N-terminal 74-amino acid domain. However, the 17K-HvTHIC interaction restricted HvTHIC targeting to chloroplasts and triggered autophagy-mediated HvTHIC degradation. Upon BYDV-GAV infection, the expression of the HvTHIC gene was significantly induced, and this was accompanied by accumulation of thiamine and salicylic acid. Silencing of HvTHIC expression promoted BYDV-GAV accumulation. Transcriptomic analysis of HvTHIC silenced and non-silenced barley plants showed that the differentially expressed genes were mainly involved in plant-pathogen interaction, plant hormone signal induction, phenylpropanoid biosynthesis, starch and sucrose metabolism, photosynthesis-antenna protein, and MAPK signaling pathway. Thiamine treatment enhanced barley resistance to BYDV-GAV. Taken together, our findings reveal a molecular mechanism underlying how BYDV impedes thiamine biosynthesis to uphold viral infection in plants.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas de Plantas / Tiamina / Hordeum Idioma: En Revista: Plant J Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Doenças das Plantas / Proteínas de Plantas / Tiamina / Hordeum Idioma: En Revista: Plant J Assunto da revista: BIOLOGIA MOLECULAR / BOTANICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China