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Temporal dynamics of apoptosis-induced proliferation in pupal wing development: implications for regenerative ability.
Ahmed-de-Prado, Sara; Estella, Carlos; Baonza, Antonio.
Afiliação
  • Ahmed-de-Prado S; The Francis Crick Institute, London, NW1 1AT, UK.
  • Estella C; Centro de Biología Molecular Severo Ochoa (CSIC/UAM), C/Nicolás Cabrera 1, Madrid, 28049, Spain.
  • Baonza A; Centro de Biología Molecular Severo Ochoa (CSIC/UAM), C/Nicolás Cabrera 1, Madrid, 28049, Spain. abaonza@cbm.csic.es.
BMC Biol ; 22(1): 98, 2024 Apr 29.
Article em En | MEDLINE | ID: mdl-38679694
ABSTRACT

BACKGROUND:

The ability of animals to regenerate damaged tissue is a complex process that involves various cellular mechanisms. As animals age, they lose their regenerative abilities, making it essential to understand the underlying mechanisms that limit regenerative ability during aging. Drosophila melanogaster wing imaginal discs are epithelial structures that can regenerate after tissue injury. While significant research has focused on investigating regenerative responses during larval stages our comprehension of the regenerative potential of pupal wings and the underlying mechanisms contributing to the decline of regenerative responses remains limited.

RESULTS:

Here, we explore the temporal dynamics during pupal development of the proliferative response triggered by the induction of cell death, a typical regenerative response. Our results indicate that the apoptosis-induced proliferative response can continue until 34 h after puparium formation (APF), beyond this point cell death alone is not sufficient to induce a regenerative response. Under normal circumstances, cell proliferation ceases around 24 h APF. Interestingly, the failure of reinitiating the cell cycle beyond this time point is not attributed to an incapacity to activate the JNK pathway. Instead, our results suggest that the function of the ecdysone-responsive transcription factor E93 is involved in limiting the apoptosis-induced proliferative response during pupal development.

CONCLUSIONS:

Our study shows that apoptosis can prolong the proliferative period of cells in the wing during pupal development as late as 34 h APF, at least 10 h longer than during normal development. After this time point, the regenerative response is diminished, a process mediated in part by the ecdysone-responsive transcription factor E93.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Pupa / Regeneração / Fatores de Transcrição / Asas de Animais / Apoptose / Proteínas de Drosophila / Proliferação de Células / Drosophila melanogaster Limite: Animals Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Pupa / Regeneração / Fatores de Transcrição / Asas de Animais / Apoptose / Proteínas de Drosophila / Proliferação de Células / Drosophila melanogaster Limite: Animals Idioma: En Revista: BMC Biol Assunto da revista: BIOLOGIA Ano de publicação: 2024 Tipo de documento: Article