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Characterization of sexual maturity-associated N6-methyladenosine in boar testes.
Zhang, Pengfei; Zhang, Fei; Sui, Heming; Yang, Xingyu; Ji, Yiming; Zheng, Shenghao; Li, Wei; Cheng, Kun; Wang, Chonglong; Jiao, Jun; Zhang, Xiaodong; Cao, Zubing; Zhang, Yunhai.
Afiliação
  • Zhang P; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Zhang F; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Sui H; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Yang X; National Animal Husbandry Service, Beijing, 100125, China.
  • Ji Y; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Zheng S; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Li W; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Cheng K; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Wang C; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China.
  • Jiao J; Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei, 230031, China.
  • Zhang X; Anhui Haoyu Animal Husbandry Co., Ltd, Luan, 237451, China.
  • Cao Z; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China. xdzhang1983@163.com.
  • Zhang Y; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, No.130 West Changjiang Road, Hefei, 230036, China. zubingcao@ahau.edu.cn.
BMC Genomics ; 25(1): 447, 2024 May 07.
Article em En | MEDLINE | ID: mdl-38714941
ABSTRACT

BACKGROUND:

The health and size of the testes are crucial for boar fertility. Testicular development is tightly regulated by epigenetics. N6-methyladenosine (m6A) modification is a prevalent internal modification on mRNA and plays an important role in development. The mRNA m6A methylation in boar testicular development still needs to be investigated.

RESULTS:

Using the MeRIP-seq technique, we identify and profile m6A modification in boar testes between piglets and adults. The results showed 7783 distinct m6A peaks in piglets and 6590 distinct m6A peaks in adults, with 2,471 peaks shared between the two groups. Enrichment of GO and KEGG analysis reveal dynamic m6A methylation in various biological processes and signalling pathways. Meanwhile, we conjointly analyzed differentially methylated and expressed genes in boar testes before and after sexual maturity, and reproductive related genes (TLE4, TSSK3, TSSK6, C11ORF94, PATZ1, PHLPP1 and PAQR7) were identified. Functional enrichment analysis showed that differential genes are associated with important biological functions, including regulation of growth and development, regulation of metabolic processes and protein catabolic processes.

CONCLUSION:

The results demonstrate that m6A methylation, differential expression and the related signalling pathways are crucial for boar testicular development. These results suggest a role for m6A modification in boar testicular development and provided a resource for future studies on m6A function in boar testicular development.
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Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Maturidade Sexual / Testículo / Adenosina Limite: Animals Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Bases de dados: MEDLINE Assunto principal: Maturidade Sexual / Testículo / Adenosina Limite: Animals Idioma: En Revista: BMC Genomics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China