Electrically Oriented Antibodies on Transistor for Monitoring Several Copies of Methylated DNA.
Anal Chem
; 96(21): 8300-8307, 2024 May 28.
Article
em En
| MEDLINE
| ID: mdl-38747393
ABSTRACT
An antibody transistor is a promising biosensing platform for the diagnosis and monitoring of various diseases. Nevertheless, the low concentration and short half-life of biomarkers require biodetection at the trace-molecule level, which remains a challenge for existing antibody transistors. Herein, we demonstrate a graphene field-effect transistor (gFET) with electrically oriented antibody probes (EOA-gFET) for monitoring several copies of methylated DNA. The electric field confines the orientation of antibody probes on graphene and diminishes the distance between graphene and methylated DNAs captured by antibodies, generating more induced charges on graphene and amplifying the electric signal. EOA-gFET realizes a limit of detection (LoD) of â¼0.12 copy µL-1, reaching the lowest LoD reported before. EOA-gFET shows a distinguishable signal for liver cancer clinical serum samples within â¼6 min, which proves its potential as a powerful tool for disease screening and diagnosis.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Transistores Eletrônicos
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Técnicas Biossensoriais
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Metilação de DNA
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Grafite
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Anticorpos
Limite:
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China