Minimal and hybrid hydrogenases are active from archaea.
Cell
; 187(13): 3357-3372.e19, 2024 Jun 20.
Article
em En
| MEDLINE
| ID: mdl-38866018
ABSTRACT
Microbial hydrogen (H2) cycling underpins the diversity and functionality of diverse anoxic ecosystems. Among the three evolutionarily distinct hydrogenase superfamilies responsible, [FeFe] hydrogenases were thought to be restricted to bacteria and eukaryotes. Here, we show that anaerobic archaea encode diverse, active, and ancient lineages of [FeFe] hydrogenases through combining analysis of existing and new genomes with extensive biochemical experiments. [FeFe] hydrogenases are encoded by genomes of nine archaeal phyla and expressed by H2-producing Asgard archaeon cultures. We report an ultraminimal hydrogenase in DPANN archaea that binds the catalytic H-cluster and produces H2. Moreover, we identify and characterize remarkable hybrid complexes formed through the fusion of [FeFe] and [NiFe] hydrogenases in ten other archaeal orders. Phylogenetic analysis and structural modeling suggest a deep evolutionary history of hybrid hydrogenases. These findings reveal new metabolic adaptations of archaea, streamlined H2 catalysts for biotechnological development, and a surprisingly intertwined evolutionary history between the two major H2-metabolizing enzymes.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Filogenia
/
Archaea
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Hidrogênio
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Hidrogenase
Idioma:
En
Revista:
Cell
Ano de publicação:
2024
Tipo de documento:
Article