Extracellular Vesicle Spherical Nucleic Acids.

Chen, Hao; Ding, Qiaojiao; Li, Lin; Wei, Pengyao; Niu, Zitong; Kong, Tong; Fu, Pan; Wang, Yuhui; Li, Jiang; Wang, Kaizhe; Zheng, Jianping

Chen, Hao; Ding, Qiaojiao; Li, Lin; Wei, Pengyao; Niu, Zitong; Kong, Tong; Fu, Pan; Wang, Yuhui; Li, Jiang; Wang, Kaizhe; Zheng, Jianping.

Afiliação

Chen H; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
Ding Q; University of Chinese Academy of Sciences, Beijing 100049, China.
Li L; Cixi Biomedical Research Institute, Wenzhou Medical University, Wenzhou 325035, China.
Wei P; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
Niu Z; Cixi Biomedical Research Institute, Wenzhou Medical University, Wenzhou 325035, China.
Kong T; Cixi Biomedical Research Institute, Wenzhou Medical University, Wenzhou 325035, China.
Fu P; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
Wang Y; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
Li J; Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315300, China.
Wang K; University of Chinese Academy of Sciences, Beijing 100049, China.
Zheng J; Institute of Materiobiology, Department of Chemistry, College of Science, Shanghai University, Shanghai 200444, China.

JACS Au ; 4(6): 2381-2392, 2024 Jun 24.

Article em En | MEDLINE | ID: mdl-38938802

ABSTRACT

ABSTRACT

Extracellular vesicles (EVs) are naturally occurring vesicles secreted by cells that can transport cargo between cells, making them promising bioactive nanomaterials. However, due to the complex and heterogeneous biological characteristics, a method for robust EV manipulation and efficient EV delivery is still lacking. Here, we developed a novel class of extracellular vesicle spherical nucleic acid (EV-SNA) nanostructures with scalability, programmability, and efficient cellular delivery. EV-SNA was constructed through the simple hydrophobic coassembly of natural EVs with cholesterol-modified oligonucleotides and can be stable for 1 month at room temperature. Based on programmable nucleic acid shells, EV-SNA can respond to AND logic gates to achieve vesicle assembly manipulation. Importantly, EV-SNA can be constructed from a wide range of biological sources EV, enhancing cellular delivery capability by nearly 10-20 times. Compared to artificial liposomal SNA, endogenous EV-SNA exhibited better biocompatibility and more effective delivery of antisense oligonucleotides in hard-to-transfect primary stem cells. Additionally, EV-SNA can deliver functional EVs for immune regulation. As a novel material form, EV-SNA may provide a modular and programmable framework paradigm for EV-based applications in drug delivery, disease treatment, nanovaccines, and other fields.

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Texto completo: 1 Bases de dados: MEDLINE Idioma: En Revista: JACS Au Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China