Genome Editing to Produce Knockout Mutations of Seed Dormancy Genes in Wheat.
Methods Mol Biol
; 2830: 137-148, 2024.
Article
em En
| MEDLINE
| ID: mdl-38977575
ABSTRACT
Knockout mutants provide definitive information about the functions of genes related to agronomic traits, including seed dormancy. However, it takes many years to produce knockout mutants using conventional techniques in polyploid plants such as hexaploid wheat. Genome editing with sequence-specific nucleases is a promising approach for obtaining knockout mutations in all targeted homoeologs of wheat simultaneously. Here, we describe a procedure to produce a triple recessive mutant in wheat via genome editing. This protocol covers the evaluation of gRNA and Agrobacterium-mediated transformation to obtain edited wheat seedlings.
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Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Triticum
/
Técnicas de Inativação de Genes
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Dormência de Plantas
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Sistemas CRISPR-Cas
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Edição de Genes
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão