Targeted Modification of Grain Dormancy Genes in Barley.
Methods Mol Biol
; 2830: 149-161, 2024.
Article
em En
| MEDLINE
| ID: mdl-38977576
ABSTRACT
Transgenesis technologies, such as overexpression or RNA interference-mediated suppression, have often been used to alter the activity of target genes. More recently developed targeted genome modification methods using customizable endonucleases allow for the regulation or knockout mutation of target genes without the necessity of integrating recombinant DNA. Such approaches make it possible to create novel alleles of target genes, thereby significantly contributing to crop improvement. Among these technologies, the Cas9 endonuclease-based method is widely applied to several crops, including barley (Hordeum vulgare). In this chapter, we describe an Agrobacterium-based approach to the targeted modification of grain dormancy genes in barley using RNA-guided Cas9 nuclease.
Palavras-chave
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
Hordeum
/
Dormência de Plantas
/
Sistemas CRISPR-Cas
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão