Fragment correlation mass spectrometry: Determining the structures of biopolymers in a complex mixture without isolating individual components.
Proc Natl Acad Sci U S A
; 121(32): e2409676121, 2024 Aug 06.
Article
em En
| MEDLINE
| ID: mdl-39074273
ABSTRACT
Fragment correlation mass spectrometry correlates ion pairs generated from the same fragmentation pathway, achieved by covariance mapping of tandem mass spectra generated with an unmodified linear ion trap without preseparation. We enable the identification of different precursors at different charge states in a complex mixture from a large isolation window, empowering an analytical approach for data-independent acquisition. The method resolves and matches isobaric fragments, internal ions, and disulfide bond fragments. We suggest that this method represents a major advance for analyzing structures of biopolymers in mixtures.
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MEDLINE
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En
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Proc Natl Acad Sci U S A
Ano de publicação:
2024
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Article