Quantification of human intravenous immunoglobulin from plasma and in process samples by affinity chromatography.
Prep Biochem Biotechnol
; : 1-6, 2024 Aug 02.
Article
em En
| MEDLINE
| ID: mdl-39091236
ABSTRACT
Advances in affinity chromatography now make it possible to analyze immunoglobulin G from plasma and its fractions with a simple chromatographic method. Ligands derived from camelid antibodies have been developed which have affinity to all 4 subclasses of human IgG without a cross reactivity to other immunoglobulins. The commercially available Capture Select FcXL is the basis for a simple method for direct quantification of immunoglobulin G from plasma or from fractions from cold ethanol precipitation. After direct injection of the sample into the column the unbound proteins are washed out with equilibration buffer and eluted with a pH-step. The elution the peak is integrated, and quantity is derived form a standard curve. The limit of detection with 40 µg/mL, and a linearity up to 250 µg/mL allows an analysis of samples ranging from 0.04 to 50 mg/mL using varying injection volume without further dilution and the two-wavelength detection. A full cycle is completed within five minutes. This method can serve as orthogonal method for in-process control but also for process development.
Texto completo:
1
Bases de dados:
MEDLINE
Idioma:
En
Revista:
Prep Biochem Biotechnol
/
Prep. biochem. biotechnol
/
Preparative biochemistry and biotechnology
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Áustria