Alterations of human endometrial stromal cells produced by N-methyl-N'-nitro-N-nitrosoguanidine.
Cancer Res
; 43(7): 3348-57, 1983 Jul.
Article
em En
| MEDLINE
| ID: mdl-6133612
ABSTRACT
Stromal cell cultures obtained from human endometrium were treated repetitively with N-methyl-N'-nitro-N-nitrosoguanidine in vitro at concentrations ranging from 0.5 to 4.0 micrograms/ml, and alterations in growth potential and morphology were analyzed. A single exposure to the carcinogen resulted in morphological evidence of toxicity and reductions in growth rates, plating efficiency, and saturation density as compared to solvent-treated control cells. Cytotoxicity was reduced after additional exposures to the carcinogen. Following repetitive treatments with N-methyl-N'-nitro-N-nitrosoguanidine, human endometrial stromal cells developed enhanced growth potential, the capacity to form macroscopic colonies in soft agar, and elevated gamma-glutamyltranspeptidase activity. Carcinogen-treated cells displayed atypical morphology characterized by irregularities in cell and nuclear size and shape, large bizarre nucleoli, increased nuclearcytoplasmic ratios, and cellular crowding. Control cells did not display altered morphology or growth parameters even following multiple exposures to solvent and repetitive subculturing. These alterations in growth potential and morphology suggest that the cells are progressing towards preneoplastic and perhaps neoplastic transformation in vitro.
Buscar no Google
Bases de dados:
MEDLINE
Assunto principal:
Endométrio
/
Metilnitronitrosoguanidina
Limite:
Female
/
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1983
Tipo de documento:
Article