Endothelial response to perfluorochemical perfusion.
Scan Electron Microsc
; (Pt 1): 311-9, 1984.
Article
em En
| MEDLINE
| ID: mdl-6740233
ABSTRACT
The purpose of this study was to evaluate, in vivo, the effect of perfluorochemical (PFC) blood substitutes on arterial endothelium following total blood replacement. Conscious-female-Sprague-Dawley rats (150-200 gms) were isovolumically exchange perfused with 3 blood volumes of PFC emulsion in an oxygen chamber. The exchange was performed at 0.5 ml/min via an indwelling-intracardiac-double-lumen catheter. One hour after the exchange, the animal was sacrificed and the circulatory system flushed free of blood and PFC emulsion with Dulbecco's solution, followed by perfusion fixation with glutaraldehyde solution. The heart, lungs and thoracic aorta were excised. The pulmonary arterial endothelial cell response was determined by scanning electron microscopy (SEM) following acute exposure to PFC emulsions under conditions of varied pH, oncotic pressure and emulsion age. These endothelial surfaces exhibited increased microvilli, leukocyte adhesion, fibrin deposition and subendothelium exposure. The magnitude of these abnormal responses varied with the degree of alkalinity and reduced oncotic pressure of the perfusate.
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Bases de dados:
MEDLINE
Assunto principal:
Artéria Pulmonar
/
Substitutos Sanguíneos
/
Endotélio
/
Fluorocarbonos
Limite:
Animals
Idioma:
En
Revista:
Scan Electron Microsc
Ano de publicação:
1984
Tipo de documento:
Article