Histochemical evaluation of delta 5,3 beta-OHSD activity in two types of porcine corpora lutea and granulosa cells in tissue culture.

Activity of delta 5,3 beta-hydroxysteroid dehydrogenase (delta 5,3-OHSD) in cultured porcine corpus luteum cells of early and middle luteal phase and granulosa cells was histochemical investigated. Control cultures as well as cultured with addition of luteinizing hormone (LH), prolactin, human chorionic gonadotrophin (HCG), and estradiol 17 beta were carried on. The stimulatory influence of LH and HCG on 3 cell types has been stated. Prolactin and estradiol stimulated only the cells of corpus luteum from early luteal phase.