Inhibition of human immunodeficiency virus type 1 (HIV) gag gene expression by an antisense oligodeoxynucleotide phosphorothioate.
Leukemia
; 9 Suppl 1: S86-8, 1995 Oct.
Article
em En
| MEDLINE
| ID: mdl-7475322
ABSTRACT
We have employed a cos-like monkey kidney cell line (B4.14) transfected with plasmids pCMVgag-pol-rre-r (containing the HIV gag and pol genes) and pCMVrev (containing the HIV rev gene), as a model to investigate whether antisense constructs could interfere with specific HIV gene and protein expression. We utilized an antisense construct (GP12A) directed against a non-regulatory region of the HIV genome, to transfect cells that expressed the above-mentioned HIV genes. Our results show that GP12A was able to attenuate levels of relevant HIV mRNA and gag proteins in the absence of cytotoxic effects.
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Bases de dados:
MEDLINE
Assunto principal:
Antivirais
/
Expressão Gênica
/
Produtos do Gene gag
/
Genes gag
/
Oligonucleotídeos Antissenso
/
HIV-1
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Leukemia
Assunto da revista:
HEMATOLOGIA
/
NEOPLASIAS
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Canadá