Application of the NASBA nucleic acid amplification method for the detection of human papillomavirus type 16 E6-E7 transcripts.
J Virol Methods
; 54(1): 75-81, 1995 Jul.
Article
em En
| MEDLINE
| ID: mdl-7559859
ABSTRACT
Using a human papillomavirus type 16 (HPV-16) E6-E7 specific primer set in a nucleic acid sequence-based amplification (NASBA) reaction, detection of HPV-16 transcripts was accomplished in a single enzymatic reaction at 41 degrees C. The NASBA reaction product was visualized either by Northern bolt analysis with an HPV-16 E6-E7-specific 32P-labelled oligonucleotide probe or by a non-radioactive enzyme-linked gel assay (ELGA). In combination with a rapid nucleic acid extraction procedure this method appears to be very suitable for the sensitive and specific detection of HPV-16 transcripts on small amounts of HPV-16-expressing cells of various sources, including cervical smears.
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Bases de dados:
MEDLINE
Assunto principal:
Papillomaviridae
/
Proteínas Repressoras
/
RNA Viral
/
Proteínas Oncogênicas Virais
/
Técnicas de Amplificação de Ácido Nucleico
Tipo de estudo:
Diagnostic_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Holanda