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Production of transforming growth factor alpha by normal human blood eosinophils.
Walz, T M; Nishikawa, B K; Malm, C; Wasteson, A.
Afiliação
  • Walz TM; Department of Cell Biology, Faculty of Health Sciences, University of Linköping, Sweden.
Leukemia ; 7(10): 1531-7, 1993 Oct.
Article em En | MEDLINE | ID: mdl-7692191
Transforming growth factor alpha (TGF-alpha) is a pleiotropic factor mediating numerous cellular responses in normal and transformed cells. This includes differentiation, proliferation, migration, and formation of extracellular matrix. TGF-alpha has been demonstrated in circulating eosinophils from the idiopathic hypereosinophilic syndrome and in differentiating promyelocytic leukemia cells in vitro. Whether TGF-alpha production also occurs in normal human blood cells is not known. Northern blot analysis showed that normal human white blood cells consistently expressed the TGF-alpha gene in 47 out of 47 donors. Cell preparations enriched in mononucleated cells, and devoid of granulocytes, showed no TGF-alpha mRNA. In situ hybridization experiments assigned the TGF-alpha gene expression to the eosinophils; 100% of the eosinophils and no other cell types were specifically recognized by the complementary human TGF-alpha riboprobe. White blood cells, incubated at 37 degrees C for up to 6 hours, released immunoreactive TGF-alpha to the incubation medium, as determined by ELISA. In contrast, no TGF-alpha protein was detected in the incubation medium of mononuclear cells. It is concluded that TGF-alpha is constitutively produced and released by normal human blood eosinophils. TGF-alpha provided by eosinophils, may participate in the inflammatory reaction by interacting with mesenchymal and epithelial cells, thus promoting fibrosis or neovascularization.
Assuntos
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Bases de dados: MEDLINE Assunto principal: Fator de Crescimento Transformador alfa / Eosinófilos Limite: Humans Idioma: En Revista: Leukemia Assunto da revista: HEMATOLOGIA / NEOPLASIAS Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Suécia
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Bases de dados: MEDLINE Assunto principal: Fator de Crescimento Transformador alfa / Eosinófilos Limite: Humans Idioma: En Revista: Leukemia Assunto da revista: HEMATOLOGIA / NEOPLASIAS Ano de publicação: 1993 Tipo de documento: Article País de afiliação: Suécia