The separation of ribonucleic acids from Escherichia coli on lysin-agarose.
Nucleic Acids Res
; 3(6): 1569-76, 1976 Jun.
Article
em En
| MEDLINE
| ID: mdl-785385
ABSTRACT
Lysine-agarose provides a simple means of separating RNA species of different moleecular weight. When RNA from Escherichia coli is added to a small column of lysine-agarose and elution is carried out at neutral pH with a shallow linear gradient of NaCl the RNA species are eluted according to size; 4S and 5S RNA species are completely separated and after a delay the 16S and 23S species are eluted as separate peaks. The process is very reproducible and the different species are eluted at a fixed salt concentration even when changes are made in the gradient, provided other conditions, under which the column is run remain constant.
Texto completo:
1
Bases de dados:
MEDLINE
Assunto principal:
RNA Bacteriano
/
Escherichia coli
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1976
Tipo de documento:
Article