Identification of GPCMV infected cells in vitro and in vivo with a monoclonal antibody.
J Virol Methods
; 48(2-3): 133-44, 1994 Jul.
Article
em En
| MEDLINE
| ID: mdl-7989431
ABSTRACT
A monoclonal antibody was made which identifies a 160-180 kDa structural protein in guinea pig cytomegalovirus (GPCMV) infected cells by Western blot using non-reducing conditions. This protein was shown to be a virion structural protein by purification of GPCMV on a density viscosity gradient and Western blot analysis. Phosphoanacetic acid (PAA) experiments suggest that the protein is a late GPCMV protein. In vitro the monoclonal antibody labels a cytoplasmic protein in infected guinea pig embryo fibroblasts by 12 h postinfection. The monoclonal antibody also identifies GPCMV infected cells in vivo in paraffin embedded formalin fixed tissue.
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Bases de dados:
MEDLINE
Assunto principal:
Virologia
/
Citomegalovirus
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
1994
Tipo de documento:
Article