Characterization of human uroguanylin: a member of the guanylin peptide family.
Am J Physiol
; 266(2 Pt 2): F342-8, 1994 Feb.
Article
em En
| MEDLINE
| ID: mdl-8141334
Guanylin, a peptide homologue of the bacterial heat-stable enterotoxins (ST), is an endogenous activator of guanylate cyclase C (GC-C). We have initiated a search for other members of the guanylin peptide family and in the current study describe a "guanylin-like peptide" from human urine. Bioactivity was monitored by determining the effect of urine extracts on T84 cell guanosine 3',5'-cyclic monophosphate (cGMP) levels. Purification yielded two bioactive peaks of peptides that, when sequenced by NH2-terminal analysis, possessed 15 and 16 amino acids. The sequence of the smaller peptide represented an NH2-terminal truncation of the larger peptide. We have termed the larger peptide human uroguanylin; it has the following amino acid sequence: NDDCELCVNVACTGCL. Human uroguanylin shares amino acid sequence homology with guanylin and ST. Synthetic uroguanylin increased cGMP levels in T84 cells, competed with 125I-labeled ST for receptors, and stimulated Cl- secretion as reflected by an increased short-circuit current. Thus we report the isolation from human urine of a unique peptide, uroguanylin, that behaves in a manner similar to guanylin and appears to be a new member of this peptide family.
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Bases de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Hormônios Gastrointestinais
Limite:
Adult
/
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Am J Physiol
Ano de publicação:
1994
Tipo de documento:
Article