High-level expression of functional cGMP-dependent protein kinase using the baculovirus system.
FEBS Lett
; 336(1): 163-7, 1993 Dec 20.
Article
em En
| MEDLINE
| ID: mdl-8262202
ABSTRACT
The understanding of the structure and function of cGMP-dependent protein kinase (cGMP kinase) has been hindered by the difficulty to obtain large quantities of functional enzyme. A recombinant baculovirus encoding bovine cGMP kinase I alpha was constructed and purified. Infected insect cells synthesized large amounts of soluble and biologically active cGMP kinase I alpha representing up to 10% of the total cell extract protein. The recombinant enzyme had an identical apparent molecular mass, cGMP affinity and kinase activity as the native bovine lung enzyme. The high-level expression of functional cGMP kinase I alpha should provide an excellent tool to study further the structure and function of cGMP kinase.
Buscar no Google
Bases de dados:
MEDLINE
Assunto principal:
Baculoviridae
/
Proteínas Quinases Dependentes de GMP Cíclico
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Alemanha