A scanning electron microscopy study of peritoneal stomata in different peritoneal regions.

ABSTRACT

Peritoneal stomata constitute the principal pathways for the drainage of intraperitoneal contents from the peritoneal cavity to the lymphatic system and have been claimed to be exclusively restricted to the peritoneal surface of the diaphragm. This concept has been revised by the demonstration of peritoneal stomata in the omental, mesenteric, ovaric and pelvic peritoneum. Therefore, the aim of this study was to further assess peritoneal surfaces of several other abdominal organs and of the abdominal wall with special reference to the occurrence of peritoneal stomata. The peritoneum covering the spleen, stomach, intestine, liver, diaphragm and anterior abdominal wall obtained from rats was examined by scanning electron microscopy. Whereas the splenic and hepatic peritoneal surfaces were composed of uniformly distributed cuboidal mesothelial cells, the gastric and intestinal peritoneal surfaces were arranged in parallel folds composed of prominent mesothelial cells with elongated finger-like cytoplasmic processes. In addition to diaphragmatic peritoneal stomata, mesothelial openings were also found on the peritoneal surfaces covering the anterior abdominal wall and the liver. The parietal peritoneal stomata were arranged in clusters, oval in shape and delimited by flattened mesothelial cells exposing the underlying submesothelial connective tissue. The hepatic mesothelial openings formed by deep channel-like gaps of adjacent cuboidal mesothelial cells were almost completely occluded by a dense microvillous coat. As the submesothelial connective tissue was not identifiable with certainty, the mesothelial openings were regarded as corresponding to stoma-like structures. These findings yield further evidence that peritoneal stomata are obviously not confined to the diaphragmatic area but extend to other peritoneal regions. It is therefore suggested that these extra-diaphragmatic parietal and visceral peritoneal surfaces contribute to the absorption capacity of the entire peritoneum and are subsequently involved in either therapeutic procedures or pathological processes affecting the peritoneal cavity.